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doi: 10.1016/J.IJBIOMAC.2024.131257 43 DongQ.GuoX.LiL.YuC.NieL.TianW.et al

Do not treat thirsty or exhausted cattle

Measurement of glutamate Cells were collected in phosphate buffer (100 mM sodium phosphate, 1 mM ethylene diamine tetraacetate, pH 7.5) and sonicated and lysates centrifuged to remove insoluble debris